Patterns of thermoregulatory mass evacuation in honey bees

Abstract

Honey bees are master thermoregulators, capable of maintaining nest homeostasis across fluctuating ambient temperatures. When workers must cool their nest, they use multiple thermoregulatory behaviors (e.g., fanning, collecting water), but bearding, where hundreds to thousands of workers evacuate their nest and form a bivouac outside, is relatively unexplored. Here, we (1) describe natural bearding patterns, (2) experimentally manipulate colonies to determine what impacts beard size and timing, and (3) explore how workers dissipate back into their nest. We show that bearding occurs daily in hot weather, but the largest beards consistently happen in the evening/night, between 1800 and 2400. Beards are located around the nest entrance, but workers bias their position towards the shaded-side of the nest box. As colony size increases, beard size and duration also increase, but the proportion of the colony bearding does not increase with colony size. Colonies with and without brood still cast beards; brood presence/absence did not impact beard size or duration. After noticing that beards tend to dissipate at sunrise, we experimentally showed that beards induced in the afternoon dissipate within 1-2 hrs, whereas beards induced in the evening remain overnight (10+ hrs). Bearding overnight, however, does carry risks for developing brood inside, as nest temperatures dropped below the optimal range, until the beard dissipated at sunrise. What cues workers use to depart the beard remain unknown, but experimentally illuminating colonies at night did not induce beards to dissipate. Our results suggest that bearding is an individual decision, not one that is coordinated across the colony. Still, these individual actions result in a dramatic collective response that colonies employ to reduce the temperature of their nest. Here, we show how and when colonies use bearding, despite its risks.