| dc.description.abstract | Platelet lysate (PL), an acellular product derived from lysed platelets, holds promise as a dual-function therapeutic with regenerative and antimicrobial properties. Rich in growth factors and antimicrobial peptides (AMPs), PL supports wound healing and disrupts bacterial membranes, offering a biologically based alternative or adjunct to antibiotics. While human and equine studies have demonstrated PL’s efficacy against pathogens such as Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, its effects on bacteria common in canine wounds remain uncharacterized. Moreover, the impact of PL on bacterial growth kinetics in canine pathogens has not been previously investigated.
This study first looked at the effects of various preparation methods on the antimicrobial activity of canine PL, considering variations in leukocyte concentration, plasma content, and complement activity. Blood from eight dogs was processed into leukocyte-rich or -reduced PRP, followed by plasma depletion or heat inactivation before generating PL. Antimicrobial activity was tested against E. coli, E. faecalis, S. pseudintermedius, and S. aureus. PL significantly reduced S. aureus and E. coli counts, while plasma depletion and complement inactivation reduced efficacy against S. pseudintermedius and E. faecalis, respectively.
Next, we investigated how PL, alone or combined with amikacin, affects bacterial growth dynamics. PL (20% and 80%) with or without amikacin was tested against S. aureus, S. pseudintermedius, and MRSA. PL reduced bacterial yield and prolonged lag time in all strains, with stronger effects at 80%. The combination with amikacin further enhanced these effects, although μₘₐₓ remained unchanged.
These findings suggest that plasma components, including complement proteins, are important contributors to the antimicrobial effects of PL against certain bacteria, whereas leukocyte concentration does not appear to play a major role. In addition, PL primarily limits bacterial proliferation and delays the onset of growth, while its combination with amikacin amplifies these effects without influencing bacterial replication speed. | en_US |
